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Databases & Software

Find molecular databases and software tools with a combined search of the HSLS Online Bioinformatics Resource Collection (OBRC) and Nucleic Acids Research.

Protocols

Videos

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  1. If you are new to miRNA study, Ambion
    Provider: Ambion
  2. Authors: Shelley Force Aldred1, Patrick Collins1, Nathan Trinklein1
  3. Describes a method for purifying miRNAs from total RNA using PAGE gel.
    Provider: Ambion
  4. Authors: Matthew J. Coussens*1, Kevin Forbes*1, Carol Kreader*1, Jack Sago1, Carrie Cupp1, John Swarthout1
  5. This protocol consists of the following procedures: 1. Oligo Design; 2. ImpA Synthesis; 3. Synthesizing Oligos; 4. Oligonucleotide Adenylation; 5. Small RNA Purification; 6. 3' Adaptor Ligation; 7. 5' Adaptor Ligation; 8. RT-PCR; 9. Concatamerization; 10. Cloning into Topo vector; 11. Screen and SequencemiRNACl...
    Provider: Bartel Lab, MIT
  6. Authors: Justin M. Wolter1,2, Kasuen Kotagama1,2, Cody S. Babb2, Marco Mangone1,2
  7. http://fatihkocabas.net/mirnacloning.aspx
    Provider: Fatih Kocabas, Moleküler Biyolog ve Genetikçi, UT Southwestern Medical Center at Dallas
  8. Authors: Marco Pacifici1, Serena Delbue2, Ferdous Kadri1, Francesca Peruzzi1
  9. Includes experimental authentication of miRNAs,
    Provider: Labome
  10. Authors: Oliver A. Kent1, Charles Steenbergen2, Samarjit Das2
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