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  1. ... Results A universal, genome-wide guide finder for CRISPR/Cas9 targeting in microbial genomes Michelle Spoto , Elizabeth ... Metrics Data Supplements Preview PDF Abstract Background: The CRISPR/Cas system has significant potential to facilitate gene ...
  2. ... animals and substantially reduce transmission. Thus, we propose the introduction of anti-betacoronavirus shRNAs using CRISPR/CAS9 gene drive into the horseshoe bat population, the natural reservoir of those viruses, to combat this pandemic thre ...
  3. ... Search for this keyword Advanced Search New Results CRISPR/Cas9 cleavages in budding yeast reveal templated insertions ... History Metrics Data Supplements Preview PDF Abstract Harnessing CRISPR-Cas9 technology has provided an unprecedented ability to ...
  4. ... Advanced Search New Results An integrated RNA and CRISPR/Cas toolkit for multiplexed synthetic circuits and endogenous ... RNA-based regulation, such as RNA interference, and CRISPR/Cas transcription factors (CRISPR-TFs), can enable scalable ...
  5. We estimate the number of spacers in a CRISPR array of a bacterium which maximizes its protection against ... an excessive variety of spacers dilutes the number of the CRISPR complexes armed with the most recent and thus most effectiv ... that depending on such parameters as the concentration of C ...
  6. ... Search for this keyword Advanced Search New Results CRISPR/Cas9-mediated resistance to cauliflower mosaic virus Haijie ... generate new and broad-spectrum antiviral strategies. The CRISPR-Cas9 (clustered regularly interspaced palindromic repeat, CRISPR-associated) ...
  7. ... this keyword Advanced Search New Results An optimized CRISPR/Cas toolbox for efficient germline and somatic genome ... Data Supplements Preview PDF Abstract The type II CRISPR/Cas system has recently emerged as a powerful ...
  8. ... Info/History Metrics Data Supplements Preview PDF Abstract CRISPR/Cas9 systems enable many molecular activities to be ... and nicks, transcriptional activation and repression, and fluorescence. CRISPR targeting relies on base pairing of short RNA ...
  9. ... binding and HEPN nuclease activation are decoupled in CRISPR-Cas13a Akshay Tambe , Alexandra East-Seletsky , View ORCID ... Info/History Metrics Data Supplements Preview PDF Abstract CRISPR-Cas13a is a CRISPR RNA (crRNA)-guided, RNA- ...
  10. CRISPR is a newly discovered prokaryotic immune system. Bacteria ... than that of the classical predator-prey model. As the CRISPR incorporates genetic material, viruses are under pressure to evolve to escape the recognition by CRISPR. When bacteria have a small rate of deleting spacers, a new ...
  11. ... Results Improved vectors and genome-wide libraries for CRISPR screening Neville E Sanjana , Ophir Shalem , Feng Zhang ... targeted loss-of-function pooled screens using the CRISPR (clustered regularly interspaced short palindrome repeats)?associated nuclease ...
  12. ... against viruses or plasmids. This system, known as the CRISPR-Cas system, allows the host to recognize and incorporate short foreign DNA or RNA sequences, called `spacers' into its CRISPR system. ... and phage population growth, spacer diversity within the CRISPR locus, and spacer protection ...
  13. ... Advanced Search New Results A versatile two-step CRISPR- and RMCE-based strategy for efficient genome engineering ... History Metrics Preview PDF Abstract The development of CRISPR/Cas9 technologies promises a quantum leap in genome- ...
  14. ... report here methods for genetic inactivation of AMPK $\alpha1/ \alpha2$ catalytic subunits in human cell lines by the CRISPR/Cas9 technology, a recent breakthrough technique for genome editing ...
  15. ... Selection of chromosomal DNA libraries using a multiplex CRISPR system in Saccharomyces cerevisiae Owen W Ryan , Jeffrey ... step and marker-free genome editing system using CRISPR-Cas9. With this Multiplex CRISPR (CRISPRm) system, we ...
  16. Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) constitute a multi-functional, ... in biotechnology. Here we review a large body of CRISPR-Cas research to explore themes of evolution and selection, ...
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