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  1. ... Results A universal, genome-wide guide finder for CRISPR/Cas9 targeting in microbial genomes Michelle Spoto , Elizabeth ... Metrics Data Supplements Preview PDF Abstract Background: The CRISPR/Cas system has significant potential to facilitate gene ...
  2. ... CRISPR has transformed the genome editing process. CRISPR could be used to modify and regulate phenomenon of our interest in organisms from all three domains of life. Applications of CRISPR mediated precision genome ... //... reprogrammed achievements. In this perspective piece, we postulate how the scientific breakthrough of CRISPR-Cas9 facilitated genome engineering could help in achieving sustainable environmental cleanup via phytoremediation ...
    Date: 1969-12-31T19:00:09-0500
  3. ... against viruses or plasmids. This system, known as the CRISPR-Cas system, allows the host to recognize and incorporate short foreign DNA or RNA sequences, called `spacers' into its CRISPR system. ... and phage population growth, spacer diversity within the CRISPR locus, and spacer protection ...
  4. The implementation of the CRISPR/Cas9 system in fission yeast by the Zaratiegui laboratory created ... and a selectable marker. The CRISPR/Cas9 system has recently been implemented in fission yeast, which allows ... resistance)-based method3,4, the pop-in/pop-out methods5, a CRISPR method based on fluoride resistance6 ...
  5. The CRISPR gene editing technology followed by next generation sequencing technology has brought up ... We present a unified GenomeSpace recipe that combines the results of a high throughput CRISPR ... Introduction The rise of next generation sequencing technology and CRISPR gene editing technology ...
  6. ... Search for this keyword Advanced Search New Results CRISPR/Cas9 cleavages in budding yeast reveal templated insertions ... History Metrics Data Supplements Preview PDF Abstract Harnessing CRISPR-Cas9 technology has provided an unprecedented ability to ...
  7. ... Clustered regularly interspersed short palindromic repeats (CRISPR) is a natural defense system for bacteria and archaea against foreign DNA and RNA. Specifically, short snippets of foreign DNA or RNA are incorporated ... //... genes into the cell for genetic engineering. Hence, plasmids are also foreign DNA with respect to the CRISPR system of the cell. To avoid destruction by the Cas protein, plasmid should not contain sequences that ...
    Date: 1969-12-31T19:00:02-0500
  8. We demonstrate that endonuclease deficient Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-associated Cas9 protein (dCas9) fused to the photo-convertible fluorescence protein monomeric mEos3.1 (dCas9-mEos3) can ...
  9. iPSCs less costly because this approach substantially reduces labour-intensive screening of CRISPR ... Human pluripotent stem cells are increasingly used for CRISPR-mediated gene targeting in efforts ... interspaced palindromic repeats (CRISPR)/Cas9, alongside continuously improving protocols for the derivation ...
  10. CRISPR Technology: Ok Conclusion: Ok, Nicely written. To summarize ... silencing (VIGS), clustered regularly interspace short palindromic repeat (CRISPR), targeting induced local ... VIGS), clustered regularly interspace short palindromic repeat (CRISPR)-Cas9 system, targeting induced ...
  11. ... Advanced Search New Results An integrated RNA and CRISPR/Cas toolkit for multiplexed synthetic circuits and endogenous ... RNA-based regulation, such as RNA interference, and CRISPR/Cas transcription factors (CRISPR-TFs), can enable scalable ...
  12. ... Recent advances in CRISPR-Cas9 genome editing tool have made great promises to basic and biomedical research as well as gene therapy. Efforts to make the CRISPR-Cas9 system applicable in gene therapy are largely ... //... consideration about the mode of DNA repair at the lesion caused by CRISPR-Cas9. In this commentary, I briefly highlight recent publications on in vivo use of the CRISPR-Cas9 system in gene therapy. I then discuss the undesired ...
    Date: 1969-12-31T19:00:29-0500
  13. binding sites due to big deletion generated after CRISPR cutting, or screening of insufficient bacterial ... cellular models of neurological disorders. Recently, however, CRISPR/Cas9 gene editing technologies have ... in the genome editing field, the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR ...
  14. The work is a confirmation that the CRISPR/Cas9 system and a template DNA design can be technically ... Background: CRISPR/Cas9 system is becoming the dominant genome editing tool in a variety ... interspaced short palindromic repeat (CRISPR), and CRISPR-associated nuclease (Cas9) - CRISPR/Cas9 are now ...
  15. ... Search for this keyword Advanced Search New Results CRISPR/Cas9-mediated resistance to cauliflower mosaic virus Haijie ... generate new and broad-spectrum antiviral strategies. The CRISPR-Cas9 (clustered regularly interspaced palindromic repeat, CRISPR-associated) ...
  16. ... pathogenic bacterium with a high genomic plasticity. Although the functional CRISPR-Cas system has not been found in its genome, CRISPR like loci have been recently identified. In this work, 53 genomes from different ... //... presence of a locus that was previously described in the VlpC gene in al lgenomes, and we characterize new CRISPR-like loci in other genomic locations. By studying the variability and gene location of these loci, the ...
    Date: 1969-12-31T19:00:12-0500
  17. CRISPR is a newly discovered prokaryotic immune system. Bacteria ... than that of the classical predator-prey model. As the CRISPR incorporates genetic material, viruses are under pressure to evolve to escape the recognition by CRISPR. When bacteria have a small rate of deleting spacers, a new ...
  18. Daniels et al. made an innovative yeast integration method based on CRISPR-cas9 system ... In this study, we developed a novel yeast integration strategy based on the widely used CRISPR-Cas9 system ... sequence is reduced. The CRISPR-Cas9 system has been extensively used to modify the genome in eukaryotes ...
  19. the mutation on the NOD background was created using CRISPR/CAS9 whereas the 3 strains where the KO ... used CRISPR to target the Zbtb32 gene for deletion directly in NOD mice and characterized the mutant ... NOD mice, we introduced a targeted deletion using CRISPR/CAS9 techniques. The creation of mutant NOD ...
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