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  1. Repeats (CRISPR-Cas9) gene knockout- (Jinek et al., 2012) strategies. Use of the sporocyst transplantation ...
  2. of the SNARE protein family. Using CRISPR/Cas9 to create a HeLa-VAMP7 knockout cell line together with knockout ... used CRISPR/Cas9 genome editing technology to generate VAMP7 knockout (KO) human HeLa cells and mouse ... editing CRISPR/Cas9 technology to generate VAMP7-knockout (KO) human HeLa cells. This genetically modified ...
  3. ... Results Improved vectors and genome-wide libraries for CRISPR screening Neville E Sanjana , Ophir Shalem , Feng Zhang ... targeted loss-of-function pooled screens using the CRISPR (clustered regularly interspaced short palindrome repeats)?associated nuclease ...
  4. ... The recent development of the CRISPR-Cas9-based gene drive has created the conditions to seriously consider this technology to solve one of the major environmental challenges in biodiversity conservation i.e. the ...
    Date: 1969-12-31T19:00:12-0500
  5. Subject: Engineering, Industrial & Manufacturing Engineering; Additive Manufacturing; Electron Beam Melting; In-Process Monitoring; Quality Control; Electronic Imaging
  6. have suggestions of essentiality in CRISPR-screening data (Sidik et al., 2016). This functional data ...
  7. such as CRISPR, into the search box results in ninety-one preprint articles culled from the aformentioned ...
  8. ... Advanced Search New Results A versatile two-step CRISPR- and RMCE-based strategy for efficient genome engineering ... History Metrics Preview PDF Abstract The development of CRISPR/Cas9 technologies promises a quantum leap in genome- ...
  9. ... rearrangements in Y. pestis. We also reconciled phylogenetic trees for each of the three CRISPR-loci to obtain an integrated scenario of the CRISPR-cassette evolution. We detected numerous parallel inversions and gain/loss events ... //... resolution of phylogenetic reconstruction. In contrast, reconstructions of strain relationships based on solely CRISPR loci may not be reliable, as the history is obscured by large deletions, obliterating the order of spacer ...
    Date: 1969-12-31T19:00:05-0500
  10. ... for deeper understanding of the coding capacity of the system. By introducing mutations in a predictable manner using CRISPR/Cas9, our technology will enable more complete investigations of cellular processes ...
  11. Subject: Life Sciences, Endocrinology & Metabolomics; calcium diet content; mineral homeostasis; skeletal homeostasis; trabecular bone; PTH(1-34); rat
  12. the ongoing malaria elimination campaign. Methods Generation of CRISPR/Cas9 pmt knock-out cell line To disrupt ...
  13. the only validation initiative put forward to identify non-specific antibodies4. CRISPR and other gene ...
  14. ... Selection of chromosomal DNA libraries using a multiplex CRISPR system in Saccharomyces cerevisiae Owen W Ryan , Jeffrey ... step and marker-free genome editing system using CRISPR-Cas9. With this Multiplex CRISPR (CRISPRm) system, we ...
  15. ... CRISPR/Cas9 is emerging as one of the most-used methods of genome modification in organisms ranging from bacteria to human cells. However, the efficiency of editing varies tremendously site-to-site. A recent report ...
    Date: 1969-12-31T19:00:20-0500
  16. ... these two parameters, our framework identifies distinct modes of immunity, including adaptive, innate, bet-hedging and CRISPR-like immunities, which recapitulate the diversity of natural immune systems ...
  17. Subject: Mathematics & Computer Science, Logic; Brocard's problem; Brocard-Ramanujan equation $x!+1=y^2$; composite Fermat numbers; Erdös' equation $x(x+1)=y!$; prime numbers of the form $n^2+1$; prime numbers of the form $n!+1$; Richert's lemma; Sophie Germain primes; Wilson primes; twin prime conjecture
  18. knock-down of Nav1.7 will not be possible, and the possibility of CRISPR-mediated gene knockout (in ... small molecules to mimic Nav1.7 gene deletion will be problematic. Nociceptor silencing through CRISPR ...
  19. genes7,8. A recent genome-scale CRISPR screen in a related apicomplexan parasite Toxoplasma gondii has ...
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