Results 21-40 of about 167|
  1. Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) constitute a multi-functional, ... in biotechnology. Here we review a large body of CRISPR-Cas research to explore themes of evolution and selection, ...
  2. Subject: Medicine & Pharmacology, Psychiatry & Mental Health Studies; schizophrenia, inflammation, nitrosative stress, tryptophan catabolites, cytokines, oxidative stress
  3. Lamc3 knockdown and knockout using Mo and CRISPR/Cas9 approaches during zebrafish development ... Methods: We use antisense morpholino oligonucleotides, and CRISPR/Cas9 mutagenesis of F0 embryos ... Here we use morpholino oligonucleotides and CRISPR/Cas9 mutagenesis of F0 embryos to characterise ...
  4. the mutation on the NOD background was created using CRISPR/CAS9 whereas the 3 strains where the KO ... used CRISPR to target the Zbtb32 gene for deletion directly in NOD mice and characterized the mutant ... NOD mice, we introduced a targeted deletion using CRISPR/CAS9 techniques. The creation of mutant NOD ...
  5. ... this keyword Advanced Search New Results An optimized CRISPR/Cas toolbox for efficient germline and somatic genome ... Data Supplements Preview PDF Abstract The type II CRISPR/Cas system has recently emerged as a powerful ...
  6. ... With the popularization of the CRISPR-Cas gene editing system there has been an explosion of new techniques made possible by this versatile technology. However, the computational field has lagged behind with a current ... //... current lack of computational tools for developing complicated CRISPR-Cas gene editing strategies. We present crseek, a Python package that provides a consistent application programming interface (API) for multiple ...
    Date: 1969-12-31T19:00:06-0500
  7. CRISPR is a newly discovered prokaryotic immune system. Bacteria ... than that of the classical predator-prey model. As the CRISPR incorporates genetic material, viruses are under pressure to evolve to escape the recognition by CRISPR. When bacteria have a small rate of deleting spacers, a new ...
  8. Subject: Engineering, Other; renewable energy; education expenditure; environmental degradation; health expenditure; carbon emissions; and foreign direct investments
  9. on to suggest that CRISPR-Cas9 will be the next natural approach to be tested and that the “optimized ... and genetically modified insects (e.g. using CRISPR-Cas9 editing), rely on the ability to consistently inject eggs ... genome editing tools such as CRISPR/Cas922. Mosquito embryo microinjection has played an integral role ...
  10. accurate than those predicted after CRISPR/CAS9 inactivation. In-silico mutation analyses of TFBSs ... CRISPR) of 10 different TF genes in K562 cells, the regulatory effects of each TF on 22,046 genes were ...
  11. ... Info/History Metrics Data Supplements Preview PDF Abstract CRISPR/Cas9 systems enable many molecular activities to be ... and nicks, transcriptional activation and repression, and fluorescence. CRISPR targeting relies on base pairing of short RNA ...
  12. ... hosts. Despite this importance, metagenomic CRISPR detection remains a major challenge. Here we present a reference-guided CRISPR spacer detection tool (Metagenomic CRISPR Reference-Aided Search Tool - MetaCRAST) that ... //... Clustered regularly interspaced short palindromic repeat (CRISPR) systems are the adaptive immune systems of bacteria and archaea against viral infection. While CRISPRs have been exploited as a tool for genetic engineering ...
    Date: 1969-12-31T19:00:19-0500
  13. We demonstrate that endonuclease deficient Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-associated Cas9 protein (dCas9) fused to the photo-convertible fluorescence protein monomeric mEos3.1 (dCas9-mEos3) can ...
  14. Subject: Social Sciences, Organizational Economics & Management; renewable energy; economic growth; public health expenditure; carbon emissions; FDI inflows; green logistics
  15. of transcription kinetics. The CRISPR/Cas9 technique was applied to alter the CTCF-binding site in the provirus ... in the provirus with CRISPR/Cas9 technique to remove CTCF from the provirus, identified the epigenetic ...
  16. the manuscript by incorporating CRISPR-Cas9 screening data analysis in the software tool. So in my opinion ... or genetic mutation using single guide RNAs (sgRNAs) with the CRISPR-Cas9 system, there is a need ... with the clustered regularly interspaced palindromic repeats-Cas9 (CRISPR-Cas9) genome editing system2,6. Pooled ...
  17. ... binding and HEPN nuclease activation are decoupled in CRISPR-Cas13a Akshay Tambe , Alexandra East-Seletsky , View ORCID ... Info/History Metrics Data Supplements Preview PDF Abstract CRISPR-Cas13a is a CRISPR RNA (crRNA)-guided, RNA- ...
  18. ... identified CRISPR spacers in the metagenomes with two different methods and found more spacers in the IC21 and C34 salterns compared with the SS19, SS33, and SS37 salterns, suggesting fewer types of CRISPR spacers ... //... their low microbial diversity, environmental stability, and high viral density. By using the power of CRISPR spacers to link viruses to their prokaryotic hosts, we explored virus-host interactions in geographically ...
    Date: 1969-12-31T19:00:14-0500
  19. ... this goal, artificial transcriptional regulators such as CRISPR-based transcription regulators have been developed ... of genetic cassettes, we could use just one synthetic CRISPR-based transcriptional regulator to simultaneously activate ...
  20. Subject: Earth Sciences, Other; SISAL database; speleothem; cave; oxygen isotopes; North America; Central America; Caribbean
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