Results 1-14 of 14 in RNA
  1. ... Advanced Search New Results An integrated RNA and CRISPR/Cas toolkit for multiplexed synthetic circuits and endogenous ... RNA-based regulation, such as RNA interference, and CRISPR/Cas transcription factors (CRISPR-TFs), can enable scalable ...
  2. ... binding and HEPN nuclease activation are decoupled in CRISPR-Cas13a Akshay Tambe , Alexandra East-Seletsky , View ORCID ... Info/History Metrics Data Supplements Preview PDF Abstract CRISPR-Cas13a is a CRISPR RNA (crRNA)-guided, RNA- ...
  3. ... Clustered regularly interspersed short palindromic repeats (CRISPR) is a natural defense system for bacteria and archaea against foreign DNA and RNA. Specifically, short snippets of foreign DNA or RNA are incorporated ... //... genes into the cell for genetic engineering. Hence, plasmids are also foreign DNA with respect to the CRISPR system of the cell. To avoid destruction by the Cas protein, plasmid should not contain sequences that ...
    Date: 1969-12-31T19:00:02-0500
  4. ... for RNA-guided gene drives based on the CRISPR nuclease Cas9 to serve as a general method ...
  5. Subject: Engineering, Biomedical & Chemical Engineering; disease classification; read mapping; feature selection; machine learning
  6. ... Info/History Metrics Data Supplements Preview PDF Abstract CRISPR/Cas9 systems enable many molecular activities to be ... and nicks, transcriptional activation and repression, and fluorescence. CRISPR targeting relies on base pairing of short RNA ...
  7. siRNA small interfering RNA, CRISPR clustered regularly interspaced short palindromic repeats, shRNA ...
  8. ... against viruses or plasmids. This system, known as the CRISPR-Cas system, allows the host to recognize and incorporate short foreign DNA or RNA sequences, called `spacers' into its CRISPR system. ... and phage population growth, spacer diversity within the CRISPR locus, and spacer protection ...
  9. ... PDF Abstract Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided nucleases have gathered considerable excitement as ... we use ChIP-seq to examine genome-wide CRISPR binding specificity at gRNA-specific and gRNA-independent ...
  10. genome RNA interference and CRISPR-based disruption screens should be cited. From ...
  11. palindromic repeat (CRISPR)/Cas-based RNA-guided DNA endonucleases21. TALEN and CRISPR/Cas are based on DNA ...
  12. ... RNA interference, or complete gene knockout, using new CRISPR techniques. These screens can reveal the basic blueprint ... framework for understanding the capabilities and limitations of CRISPR technology, we derive gold-standard reference sets of ...
  13. ... rearrangements in Y. pestis. We also reconciled phylogenetic trees for each of the three CRISPR-loci to obtain an integrated scenario of the CRISPR-cassette evolution. We detected numerous parallel inversions and gain/loss events ... //... resolution of phylogenetic reconstruction. In contrast, reconstructions of strain relationships based on solely CRISPR loci may not be reliable, as the history is obscured by large deletions, obliterating the order of spacer ...
    Date: 1969-12-31T19:00:05-0500