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  1. ... Results A universal, genome-wide guide finder for CRISPR/Cas9 targeting in microbial genomes Michelle Spoto , Elizabeth ... Metrics Data Supplements Preview PDF Abstract Background: The CRISPR/Cas system has significant potential to facilitate gene ...
  2. ... CRISPR has transformed the genome editing process. CRISPR could be used to modify and regulate phenomenon of our interest in organisms from all three domains of life. Applications of CRISPR mediated precision genome ... //... reprogrammed achievements. In this perspective piece, we postulate how the scientific breakthrough of CRISPR-Cas9 facilitated genome engineering could help in achieving sustainable environmental cleanup via phytoremediation ...
    Date: 1969-12-31T19:00:09-0500
  3. Subject: Physical Sciences, Condensed Matter Physics; dynamical equation of period vectors, crystal structure prediction, microscopic equations of state, thermodynamical properties, system collapse, external stress
  4. Rodriguez-Lopez and colleagues report improvements on CRISPR/Cas9-mediated genome editing in S ... and a selectable marker. The CRISPR/Cas9 system has recently been implemented in fission yeast, which allows ... resistance)-based method3,4, the pop-in/pop-out methods5, a CRISPR method based on fluoride resistance6 ...
  5. We present a unified GenomeSpace recipe that combines the results of a high throughput CRISPR ... Introduction The rise of next generation sequencing technology and CRISPR gene editing technology ...
  6. Features of the CRISPR-Cas system, in which bacteria integrate small segments of ... Emphasizing the population-level impact of the CRISPR-Cas system, recent experiments show that some bacteria regulate CRISPR-associated genes via the quorum sensing (QS) pathway. Here ...
  7. ... Clustered regularly interspersed short palindromic repeats (CRISPR) is a natural defense system for bacteria and archaea against foreign DNA and RNA. Specifically, short snippets of foreign DNA or RNA are incorporated ... //... genes into the cell for genetic engineering. Hence, plasmids are also foreign DNA with respect to the CRISPR system of the cell. To avoid destruction by the Cas protein, plasmid should not contain sequences that ...
    Date: 1969-12-31T19:00:02-0500
  8. ... Search for this keyword Advanced Search New Results CRISPR/Cas9 cleavages in budding yeast reveal templated insertions ... History Metrics Data Supplements Preview PDF Abstract Harnessing CRISPR-Cas9 technology has provided an unprecedented ability to ...
  9. CRISPR Technology: Ok Conclusion: Ok, Nicely written. To summarize ... silencing (VIGS), clustered regularly interspace short palindromic repeat (CRISPR), targeting induced local ... VIGS), clustered regularly interspace short palindromic repeat (CRISPR)-Cas9 system, targeting induced ...
  10. binding sites due to big deletion generated after CRISPR cutting, or screening of insufficient bacterial ... cellular models of neurological disorders. Recently, however, CRISPR/Cas9 gene editing technologies have ... in the genome editing field, the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR ...
  11. We estimate the number of spacers in a CRISPR array of a bacterium which maximizes its protection against ... an excessive variety of spacers dilutes the number of the CRISPR complexes armed with the most recent and thus most effectiv ... that depending on such parameters as the concentration of C ...
  12. Subject: Earth Sciences, Atmospheric Science; evaporation; moisture budget; precipitation; recycling ratio
  13. ... Advanced Search New Results An integrated RNA and CRISPR/Cas toolkit for multiplexed synthetic circuits and endogenous ... RNA-based regulation, such as RNA interference, and CRISPR/Cas transcription factors (CRISPR-TFs), can enable scalable ...
  14. ... against viruses or plasmids. This system, known as the CRISPR-Cas system, allows the host to recognize and incorporate short foreign DNA or RNA sequences, called `spacers' into its CRISPR system. ... and phage population growth, spacer diversity within the CRISPR locus, and spacer protection ...
  15. The work is a confirmation that the CRISPR/Cas9 system and a template DNA design can be technically ... Background: CRISPR/Cas9 system is becoming the dominant genome editing tool in a variety ... interspaced short palindromic repeat (CRISPR), and CRISPR-associated nuclease (Cas9) - CRISPR/Cas9 are now ...
  16. Daniels et al. made an innovative yeast integration method based on CRISPR-cas9 system ... In this study, we developed a novel yeast integration strategy based on the widely used CRISPR-Cas9 system ... sequence is reduced. The CRISPR-Cas9 system has been extensively used to modify the genome in eukaryotes ...
  17. ... Recent advances in CRISPR-Cas9 genome editing tool have made great promises to basic and biomedical research as well as gene therapy. Efforts to make the CRISPR-Cas9 system applicable in gene therapy are largely ... //... consideration about the mode of DNA repair at the lesion caused by CRISPR-Cas9. In this commentary, I briefly highlight recent publications on in vivo use of the CRISPR-Cas9 system in gene therapy. I then discuss the undesired ...
    Date: 1969-12-31T19:00:29-0500
  18. Subject: Medicine & Pharmacology, Veterinary Medicine; Zoonoses, food-borne, disease control, public health, domestic livestock, pigs, One health
  19. Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) constitute a multi-functional, ... in biotechnology. Here we review a large body of CRISPR-Cas research to explore themes of evolution and selection, ...
  20. the mutation on the NOD background was created using CRISPR/CAS9 whereas the 3 strains where the KO ... used CRISPR to target the Zbtb32 gene for deletion directly in NOD mice and characterized the mutant ... NOD mice, we introduced a targeted deletion using CRISPR/CAS9 techniques. The creation of mutant NOD ...
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