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  1. ... this keyword Advanced Search New Results An optimized CRISPR/Cas toolbox for efficient germline and somatic genome ... Data Supplements Preview PDF Abstract The type II CRISPR/Cas system has recently emerged as a powerful ...
  2. ... With the popularization of the CRISPR-Cas gene editing system there has been an explosion of new techniques made possible by this versatile technology. However, the computational field has lagged behind with a current ... //... current lack of computational tools for developing complicated CRISPR-Cas gene editing strategies. We present crseek, a Python package that provides a consistent application programming interface (API) for multiple ...
    Date: 1969-12-31T19:00:06-0500
  3. CRISPR is a newly discovered prokaryotic immune system. Bacteria ... than that of the classical predator-prey model. As the CRISPR incorporates genetic material, viruses are under pressure to evolve to escape the recognition by CRISPR. When bacteria have a small rate of deleting spacers, a new ...
  4. on to suggest that CRISPR-Cas9 will be the next natural approach to be tested and that the “optimized ... and genetically modified insects (e.g. using CRISPR-Cas9 editing), rely on the ability to consistently inject eggs ... genome editing tools such as CRISPR/Cas922. Mosquito embryo microinjection has played an integral role ...
  5. the manuscript by incorporating CRISPR-Cas9 screening data analysis in the software tool. So in my opinion ... or genetic mutation using single guide RNAs (sgRNAs) with the CRISPR-Cas9 system, there is a need ... with the clustered regularly interspaced palindromic repeats-Cas9 (CRISPR-Cas9) genome editing system2,6. Pooled ...
  6. ... Info/History Metrics Data Supplements Preview PDF Abstract CRISPR/Cas9 systems enable many molecular activities to be ... and nicks, transcriptional activation and repression, and fluorescence. CRISPR targeting relies on base pairing of short RNA ...
  7. ... hosts. Despite this importance, metagenomic CRISPR detection remains a major challenge. Here we present a reference-guided CRISPR spacer detection tool (Metagenomic CRISPR Reference-Aided Search Tool - MetaCRAST) that ... //... Clustered regularly interspaced short palindromic repeat (CRISPR) systems are the adaptive immune systems of bacteria and archaea against viral infection. While CRISPRs have been exploited as a tool for genetic engineering ...
    Date: 1969-12-31T19:00:19-0500
  8. ... against viruses or plasmids. This system, known as the CRISPR-Cas system, allows the host to recognize and incorporate short foreign DNA or RNA sequences, called `spacers' into its CRISPR system. ... and phage population growth, spacer diversity within the CRISPR locus, and spacer protection ...
  9. of transcription kinetics. The CRISPR/Cas9 technique was applied to alter the CTCF-binding site in the provirus ... in the provirus with CRISPR/Cas9 technique to remove CTCF from the provirus, identified the epigenetic ...
  10. accurate than those predicted after CRISPR/CAS9 inactivation. In-silico mutation analyses of TFBSs ... CRISPR) of 10 different TF genes in K562 cells, the regulatory effects of each TF on 22,046 genes were ...
  11. ... binding and HEPN nuclease activation are decoupled in CRISPR-Cas13a Akshay Tambe , Alexandra East-Seletsky , View ORCID ... Info/History Metrics Data Supplements Preview PDF Abstract CRISPR-Cas13a is a CRISPR RNA (crRNA)-guided, RNA- ...
  12. ... CRISPR/Cas9 technology enables the rapid and efficient generation of total loss-of- function mutations in a targeted gene in mammalian cells. A single cell that harbors those mutations can be used to establish a ... //... a new cell line, thereby creating a CRISPR-induced knockout clone. These clonal cell lines serve as crucial tools for exploring protein function, analyzing the consequences of gene loss, and investigating the specificity ...
    Date: 1969-12-31T19:00:31-0500
  13. ... this goal, artificial transcriptional regulators such as CRISPR-based transcription regulators have been developed ... of genetic cassettes, we could use just one synthetic CRISPR-based transcriptional regulator to simultaneously activate ...
  14. Repeats (CRISPR-Cas9) gene knockout- (Jinek et al., 2012) strategies. Use of the sporocyst transplantation ...
  15. of the SNARE protein family. Using CRISPR/Cas9 to create a HeLa-VAMP7 knockout cell line together with knockout ... used CRISPR/Cas9 genome editing technology to generate VAMP7 knockout (KO) human HeLa cells and mouse ... editing CRISPR/Cas9 technology to generate VAMP7-knockout (KO) human HeLa cells. This genetically modified ...
  16. ... Results Improved vectors and genome-wide libraries for CRISPR screening Neville E Sanjana , Ophir Shalem , Feng Zhang ... targeted loss-of-function pooled screens using the CRISPR (clustered regularly interspaced short palindrome repeats)?associated nuclease ...
  17. We speculate that there are connections between hormonal changes, the frequency of DNA methylation, and disease. The microbiome may also affect the production of those hormones. Short Chain Fatty Acids as butyrate, propionate, folate, and acetate act as ligands...
    Date: 1969-12-31T19:00:30-0500
  18. have suggestions of essentiality in CRISPR-screening data (Sidik et al., 2016). This functional data ...
  19. such as CRISPR, into the search box results in ninety-one preprint articles culled from the aformentioned ...
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