Results 1-8 of 8 in Genome Engineering
  1. ... Clustered regularly interspersed short palindromic repeats (CRISPR) is a natural defense system for bacteria and archaea against foreign DNA and RNA. Specifically, short snippets of foreign DNA or RNA are incorporated ... //... genes into the cell for genetic engineering. Hence, plasmids are also foreign DNA with respect to the CRISPR system of the cell. To avoid destruction by the Cas protein, plasmid should not contain sequences that ...
    Date: 1969-12-31T19:00:02-0500
  2. We speculate that there are connections between hormonal changes, the frequency of DNA methylation, and disease. The microbiome may also affect the production of those hormones. Short Chain Fatty Acids as butyrate, propionate, folate, and acetate act as ligands...
    Date: 1969-12-31T19:00:30-0500
  3. ... Advanced Search New Results A versatile two-step CRISPR- and RMCE-based strategy for efficient genome engineering ... History Metrics Preview PDF Abstract The development of CRISPR/Cas9 technologies promises a quantum leap in genome- ...
  4. ... this keyword Advanced Search New Results An optimized CRISPR/Cas toolbox for efficient germline and somatic genome ... Data Supplements Preview PDF Abstract The type II CRISPR/Cas system has recently emerged as a powerful ...
  5. on to suggest that CRISPR-Cas9 will be the next natural approach to be tested and that the “optimized ... and genetically modified insects (e.g. using CRISPR-Cas9 editing), rely on the ability to consistently inject eggs ... genome editing tools such as CRISPR/Cas922. Mosquito embryo microinjection has played an integral role ...
  6. ... CRISPR has transformed the genome editing process. CRISPR could be used to modify and regulate phenomenon of our interest in organisms from all three domains of life. Applications of CRISPR mediated precision genome ... //... reprogrammed achievements. In this perspective piece, we postulate how the scientific breakthrough of CRISPR-Cas9 facilitated genome engineering could help in achieving sustainable environmental cleanup via phytoremediation ...
    Date: 1969-12-31T19:00:09-0500
  7. of the SNARE protein family. Using CRISPR/Cas9 to create a HeLa-VAMP7 knockout cell line together with knockout ... used CRISPR/Cas9 genome editing technology to generate VAMP7 knockout (KO) human HeLa cells and mouse ... editing CRISPR/Cas9 technology to generate VAMP7-knockout (KO) human HeLa cells. This genetically modified ...
  8. ... hosts. Despite this importance, metagenomic CRISPR detection remains a major challenge. Here we present a reference-guided CRISPR spacer detection tool (Metagenomic CRISPR Reference-Aided Search Tool - MetaCRAST) that ... //... Clustered regularly interspaced short palindromic repeat (CRISPR) systems are the adaptive immune systems of bacteria and archaea against viral infection. While CRISPRs have been exploited as a tool for genetic engineering ...
    Date: 1969-12-31T19:00:19-0500