Results 1-20 of 23 in Cell
  1. ... CRISPR/Cas9 technology enables the rapid and efficient generation of total loss-of- function mutations in a targeted gene in mammalian cells. A single cell that harbors those mutations can be used to establish a ... //... a new cell line, thereby creating a CRISPR-induced knockout clone. These clonal cell lines serve as crucial tools for exploring protein function, analyzing the consequences of gene loss, and investigating the specificity ...
    Date: 1969-12-31T19:00:31-0500
  2. the ongoing malaria elimination campaign. Methods Generation of CRISPR/Cas9 pmt knock-out cell line To disrupt ...
  3. 2013). Furthermore, a recent genome-wide CRISPR/Cas9 screen indicated a relatively minor contribution ...
  4. of the SNARE protein family. Using CRISPR/Cas9 to create a HeLa-VAMP7 knockout cell line together with knockout ... used CRISPR/Cas9 genome editing technology to generate VAMP7 knockout (KO) human HeLa cells and mouse ... editing CRISPR/Cas9 technology to generate VAMP7-knockout (KO) human HeLa cells. This genetically modified ...
  5. ... valid cellular model to study the disease, MUT gene was knocked out (KO) in HEK293 cell line by using CRISPR-CAS9 technology. Methylmalonic acid was measured in MUT-KO and wild type (WT) cells by multiple reaction ...
    Date: 1969-12-31T19:00:09-0500
  6. ... Clustered regularly interspersed short palindromic repeats (CRISPR) is a natural defense system for bacteria and archaea against foreign DNA and RNA. Specifically, short snippets of foreign DNA or RNA are incorporated ... //... genes into the cell for genetic engineering. Hence, plasmids are also foreign DNA with respect to the CRISPR system of the cell. To avoid destruction by the Cas protein, plasmid should not contain sequences that ...
    Date: 1969-12-31T19:00:02-0500
  7. The work is a confirmation that the CRISPR/Cas9 system and a template DNA design can be technically ... Background: CRISPR/Cas9 system is becoming the dominant genome editing tool in a variety ... interspaced short palindromic repeat (CRISPR), and CRISPR-associated nuclease (Cas9) - CRISPR/Cas9 are now ...
  8. ... for deeper understanding of the coding capacity of the system. By introducing mutations in a predictable manner using CRISPR/Cas9, our technology will enable more complete investigations of cellular processes ...
  9. the mutation on the NOD background was created using CRISPR/CAS9 whereas the 3 strains where the KO ... used CRISPR to target the Zbtb32 gene for deletion directly in NOD mice and characterized the mutant ... NOD mice, we introduced a targeted deletion using CRISPR/CAS9 techniques. The creation of mutant NOD ...
  10. cells have been edited by CRISPR and could be used to the same ends possibly more easily. Further ...
  11. binding sites due to big deletion generated after CRISPR cutting, or screening of insufficient bacterial ... cellular models of neurological disorders. Recently, however, CRISPR/Cas9 gene editing technologies have ... in the genome editing field, the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR ...
  12. palindromic repeat (CRISPR)/Cas-based RNA-guided DNA endonucleases21. TALEN and CRISPR/Cas are based on DNA ...
  13. accurate than those predicted after CRISPR/CAS9 inactivation. In-silico mutation analyses of TFBSs ... CRISPR) of 10 different TF genes in K562 cells, the regulatory effects of each TF on 22,046 genes were ...
  14. 91. Additional biomarkers associated with apoptosis, necrosis and possible interactions with CRISPR complex ...
  15. ... CRISPR/Cas9 is emerging as one of the most-used methods of genome modification in organisms ranging from bacteria to human cells. However, the efficiency of editing varies tremendously site-to-site. A recent report ...
    Date: 1969-12-31T19:00:20-0500
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