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Databases/Tools

Find molecular databases and software tools with a combined search of the
HSLS Online Bioinformatics Resource Collection (OBRC) and the BioMed Central Databases collection.
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Articles on Databases/Tools

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Genes/Proteins

Find information on genes and proteins with a combined search of NCBI Entrez Gene and the BIOBASE Knowledge Library*.
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Pathways

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the Protein Lounge Pathway Database* and the Science STKE Database of Cell Signaling*.

Web

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Results 1-20 of about 129|
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  1. Resources miRNAnew windowpreview
    If you are new to miRNA study, Ambion
    Provider: Ambion
    Protocol Online
  2. Construction of Parallel Analysis of RNA Ends (PARE) libraries for the study of cleaved miRNA targets and the RNA degrad...new windowpreview
    Author: Marcelo A German, Shujun Luo, Gary Schroth, Blake C Meyers & Pamela J Green
    Affiliation: Delaware Biotechnology Institute, University of Delaware
    Access: PITT-UPMC Users Only
    Nature Protocols
  3. PROTOCOL: Detection of si/miRNA in C. elegans by Northern Blotnew windowpreview
    ......Protocol 176 107 160 168 203 88 Detection of si/miRNA in C. elegans by Northern Blot This protocol was adapted...also be detected by RNase protection (see Detection of si/miRNA in C. elegans Using RNase Protection ). MATERIALS Reagents......
    Author: René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk
    Source: CSH Protocols; 2006; doi:10.1101/pdb.prot4320
    Access: PITT-UPMC Users Only
    Cold Spring Harbor Protocols
  4. Identification of microRNAs and other small regulatory RNAs using cDNA library sequencingnew windowpreview
    Journal: Methods, Volume 44, Issue 1, January 2008, Pages 3-12
    Author: Markus Hafner, Pablo Landgraf, Janos Ludwig, Amanda Rice, Tolulope Ojo, Carolina Lin, Daniel Holoch, Cindy Lim, Thomas Tuschl
    Access: PITT-UPMC Users Only
    Methods
  5. Chapter 17 Extension of Endogenous Primers as a Tool to Detect Micro-RNA Targetsnew windowpreview
    Journal: Methods in Enzymology, Volume 449, 2008, Pages 357-371
    Author: Sergei Vatolin, Robert J. Weil
    Access: PITT-UPMC Users Only
    Methods in Enzymology
  6. Prediction of Plant miRNA Genesnew windowpreview
    Summary: Prediction of Plant miRNA Genes This chapter presents procedures for the computational identification of plant miRNA genes. In the first procedure, homologs of known miRNAs are identified...
    Published: Jan-01-2009
    Springer Protocols
  7. Gel Purification of miRNA from Total RNA new windowpreview
    Describes a method for purifying miRNAs from total RNA using PAGE gel.
    Provider: Ambion
    Protocol Online
  8. MicroRNA expression profiling using microarraysnew windowpreview
    Author: Chang-Gong Liu, George Adrian Calin, Stefano Volinia & Carlo M. Croce
    Affiliation: Department of Molecular Virology, Immunology and Medical Genetics and Comprehensive Cancer Center, The Ohio State University
    Access: PITT-UPMC Users Only
    Nature Protocols
  9. PROTOCOL: Detection of siRNA in C. elegans Using RNase Protectionnew windowpreview
    ......Caenorhabditis elegans (see Isolation of RNA from C. elegans ). siRNAs can also be detected by northern blot (see Detection of si/miRNA in C. elegans by Northern Blot ). However, the RNase protection assay seems to be more sensitive. MATERIALS Reagents......
    Author: René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk
    Source: CSH Protocols; 2006; doi:10.1101/pdb.prot4319
    Access: PITT-UPMC Users Only
    Cold Spring Harbor Protocols
  10. Expression profiling of microRNA using oligo DNA arraysnew windowpreview
    Journal: Methods, Volume 44, Issue 1, January 2008, Pages 22-30
    Author: Chang-Gong Liu, Riccardo Spizzo, George Adrian Calin, Carlo Maria Croce
    Access: PITT-UPMC Users Only
    Methods
  11. Chapter 18 Examining the Influence of MicroRNAs on Translation Efficiency and on mRNA Deadenylation and Decaynew windowpreview
    Journal: Methods in Enzymology, Volume 449, 2008, Pages 373-393
    Author: Ligang Wu, Joel G. Belasco
    Access: PITT-UPMC Users Only
    Methods in Enzymology
  12. miRNA Target Prediction in Plantsnew windowpreview
    Summary: miRNA Target Prediction in Plants In plants, miRNA bind to target RNAs with a high degree of complementarity. In this chapter, a simple method for computationally predicting plant miRNA targets...
    Published: Jan-01-2009
    Springer Protocols
  13. MicroRNA (miRNA) and siRNA Cloning Protocol new windowpreview
    This protocol consists of the following procedures: 1. Oligo Design; 2. ImpA Synthesis; 3. Synthesizing Oligos; 4. Oligonucleotide Adenylation; 5. Small RNA Purification; 6. 3' Adaptor Ligation; 7. 5' Adaptor Ligation; 8. RT-PCR; 9. Concatamerization; 10. Cloning into Topo vector; 11. Screen and SequencemiRNACl...
    Provider: Bartel Lab, MIT
    Protocol Online
  14. A methodology for the combined in situ analyses of the precursor and mature forms of microRNAs and correlation with thei...new windowpreview
    Author: Gerard J Nuovo, Terry S Elton, Patrick Nana-Sinkam, Stefano Volinia, Carlo M Croce et al.
    Affiliation: Department of Pathology, Ohio State University Medical Center
    Access: PITT-UPMC Users Only
    Nature Protocols
  15. PROTOCOL: DNA Extraction from Formalin-Fixed, Paraffin-Embedded Tissuenew windowpreview
    ......Smyth, P., Flavin, R., Cahill, S., Denning, K., Aherne, S., Guenther, S.M., OLeary, J.J., and Sheils, O. 2007. Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and......
    Author: Weining Tang, Freedom B. David, Malania M. Wilson, Benjamin G. Barwick, Brian R. Leyland-Jones, and Mark M. Bouzyk
    Source: CSH Protocols; 2009; doi:10.1101/pdb.prot5138
    Access: PITT-UPMC Users Only
    Cold Spring Harbor Protocols
  16. Real-time PCR quantification of precursor and mature microRNAnew windowpreview
    Journal: Methods, Volume 44, Issue 1, January 2008, Pages 31-38
    Author: Thomas D. Schmittgen, Eun Joo Lee, Jinmai Jiang, Anasuya Sarkar, Liuqing Yang, Terry S. Elton, Caifu Chen
    Access: PITT-UPMC Users Only
    Methods
  17. Identification of Viral MicroRNAsnew windowpreview
    Journal: Methods in Enzymology, Volume 427, 2007, Pages 1, 3-23
    Author: Christopher S. Sullivan, Adam Grundhoff
    Access: PITT-UPMC Users Only
    Methods in Enzymology
  18. An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAsnew windowpreview
    Summary: . Furthermore, the fly’s relatively simple genome appears to contain fewer cases of paralogous and hence, fewer redundant, miRNA genes than more complex vertebrate genomes. Indeed, genetic knockouts...
    Published: Dec-14-2007
    Springer Protocols
  19. Cloning Protocol miRNAnew windowpreview
    http://fatihkocabas.net/mirnacloning.aspx
    Provider: Fatih Kocabas, Moleküler Biyolog ve Genetikçi, UT Southwestern Medical Center at Dallas
    Protocol Online
  20. Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes ...new windowpreview
    Author: Asli N Silahtaroglu, Dorrit Nolting, Lars Dyrskjot...Morten Moller, Niels Tommerup, Sakari Kauppinen
    Affiliation: Wilhelm Johannsen Centre for Functional Genome Research, Department of Cellular and Molecular Medicine, University of Copenhagen, Blegdamsvej 3
    Access: PITT-UPMC Users Only
    Nature Protocols
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